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HelpTest Code LAB878 HIV-1 Ultra Sensitive RNA Quantitation by PCR
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HIV1UL
PERFORMING SITE
Pathology Laboratory
Laboratory Section: Microbiology
SPECIMEN COLLECTION
Container/Tube Type: Lavender (EDTA)
Collection Information: Collect blood in a lavender (EDTA) tube.
Transport Information: Centrifuge and transfer 1.4 mL plasma to a plastic transport tube. Send refrigerated.
Remarks: Remove plasma from cells within 24 hours and transfer to a polypropylene tube at 2-8°C for transport.
CPT Codes
87536
Minimum Volume
1.0 mL of plasma (approximately 2.0 mL of whole blood based upon a normal patient hematocrit)
Specimen Stability
Stability Comments: Freeze thaw studies show specimen may be frozen and thawed up to 4 times without loss of HIV-1 RNA.
Stability
Refrigerated Stability:
Whole blood in EDTA - 24 hours
Plasma - 6 days
Frozen Stability:
Plasma - ≤ -18° C - 12 weeks
Plasma - ≤ -60° C - 6 months
Ambient Stability: Whole blood in EDTA - 24 hours
Reject Due To
Unacceptable Conditions: Serum and heparinized samples. ACD plasma will yield 15% lower test results than EDTA plasma.
Method
Polymerase Chain Reaction
Notes
Performed: Thursday
Analytical Time: Within 8 Days
Alias
Acquired Immune Deficiency Syndrome (AIDS), AIDS (Acquired Immune Deficiency Syndrome), HIV (Human Immunodeficiency Virus), HIV-1 (Human Immunodeficiency Virus-1) RNA, Viral Load, HIV-1 PCR, HIV-1 Quantitation, PCR, Human Immunodeficiency Virus (HIV), Human Immunodeficiency Virus, PCR, PCR, HIV-1 Quantitation, Viral Load, Viral Load, HIV-1 RNA