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HelpTest Code LAB7004 Protein, Total, Body Fluid
Additional Codes
SQ: TPBFM
MAYO: TPBF
Reporting Name
Protein, Total, BFPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Body FluidOrdering Guidance
For protein measurement in spinal fluid specimens, order TPSF / Protein, Total, Spinal Fluid. Testing will be changed to TPSF if this test is ordered on that specimen type.
Necessary Information
1. Date and time of collection are required.
2. Specimen source is required.
Specimen Required
Specimen Type: Body fluid
Preferred Source:
-Peritoneal fluid (peritoneal, abdominal, ascites, paracentesis)
-Pleural fluid (pleural, chest, thoracentesis)
-Drain fluid (drainage, JP drain)
-Pericardial
Acceptable Source: Write in source name with source location (if appropriate)
Collection Container/Tube: Sterile container
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge to remove any cellular material and transfer into a plastic vial.
2. Indicate the specimen source and source location on label.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Body Fluid | Refrigerated (preferred) | 7 days | |
| Frozen | 30 days | ||
| Ambient | 24 hours |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Sunday
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84157
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TPBF | Protein, Total, BF | 2881-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TPBF1 | Protein, Total, BF | 2881-1 |
| FLD23 | Fluid Type, Protein, Total | 14725-6 |
Method Description
Divalent copper reacts in alkaline solution with protein peptide bonds to form the characteristic purple-colored biuret complex. Sodium potassium tartrate prevents the precipitation of copper hydroxide and potassium iodide prevents auto-reduction of copper. The color intensity is directly proportional to the protein concentration which can be determined photometrically.(Package insert: Protein. Roche Diagnostics; 03/2022)
Report Available
Same day/1 to 2 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Anticoagulant or additive Breast milk Nasal secretions Gastric secretions Bronchoalveolar lavage (BAL) or bronchial washings Colostomy/ostomy Feces Cerebrospinal fluid Saliva Sputum Urine Vitreous fluid |
Reject |
Method Name
Colorimetric
Useful For
Identification of exudative pleural effusions
Differentiating hepatic from other causes of ascites that have elevated serum ascites albumin gradient using peritoneal fluid
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.