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Test Code LAB693 Bile Acids, Total, Serum

Additional Codes

SQ: BILEB

MAYO: BILEA

Secondary ID

84689

Useful For

An aid in the evaluation of liver function

 

Evaluation of liver function changes before the formation of more advanced clinical signs of illness such as icterus

 

An aid in the determination of hepatic dysfunction as a result of chemical and environmental injury

 

An indicator of hepatic histological improvement in chronic hepatitis C patients responding to interferon treatment

 

An indicator for intrahepatic cholestasis of pregnancy

Method Name

Enzymatic

Reporting Name

Bile Acids, Total, S

Specimen Type

Serum


Ordering Guidance


This test is for evaluation of hepatobiliary dysfunction.

 

For evaluation of bowel dysfunction, order BA48F / Bile Acids, Bowel Dysfunction, 48 Hour, Feces.

For evaluation of patients treated with urso or cholate, order BAFS / Bile Acids, Fractionated and Total, Serum.

For evaluation of inborn errors of metabolism, order BAIPD / Bile Acids for Peroxisomal Disorders, Serum.



Specimen Required


Patient Preparation: Patient must be fasting for 12 hours. Infants and pregnant patients do not need to fast.

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and serum aliquoted into plastic vial within 2 hours of collection.


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  30 days
  Ambient  24 hours

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus Reject

Reference Values

≤10 mcmol/L

 

Reference interval applies to fasting total bile acid concentrations.

Method Description

Testing is performed on the Roche cobas c502. In the presence of thionicotinamide adenine dinucleotide (Thio-NAD), the enzyme 3-alpha-hydroxysteroid dehydrogenase (3-alpha-HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible, and 3-alpha-HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 405 nm. (Package insert: Total Bile Acids Assay Kit. Diazyme Laboratories; 01/2020)

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82239

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BILEA Bile Acids, Total, S 14628-2

 

Result ID Test Result Name Result LOINC Value
BILEA Bile Acids, Total, S 14628-2

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request (T728) with the specimen.