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Test Code LAB5110 Disaccharidase Activity Panel, Tissue

Additional Codes

SQ: FDSACM

MAYO: DSAC


Shipping Instructions


Freeze immediately after collection and ship frozen.



Specimen Required


Specimen Type: Tissue

Source: Intestinal biopsy

Supplies: 15 mL Tissue Tube-DSAC (T993)

Container/Tube: 15 mL tissue tube

Specimen Volume: 5 mg

Collection Instructions:

1. Place specimen in tissue tube.

2. Specimen should not be placed on gauze, filter paper, or swabs/wooden sticks and should not have any saline, water, support, or embedding material added.


Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Biochemical Genetics Patient Information (T602)

Gastroenterology and Hepatology Test Request (T728)

Secondary ID

608236

Useful For

Evaluation of patients who present with signs or symptoms suggestive of disaccharidase disorders

 

This test is not intended for carrier detection.

Genetics Test Information

Diagnostic testing for patients with clinical signs and symptoms suspicious of a disaccharidase deficiency.

Method Name

Spectrophotometry

Reporting Name

Disaccharidase Activity Panel, Ts

Specimen Type

Tissue

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Frozen 28 days OTHER

Reject Due To

Specimens sent in formaldehyde, saline, or any supportive media
Specimens placed on gauze, filter paper, foil, or swabs/wooden sticks		 Reject

Reference Values

Lactase: ≥14.0 nmol/min/mg protein

Sucrase: ≥19.0 nmol/min/mg protein

Maltase: ≥70.0 nmol/min/mg protein

Palatinase: ≥6.0 nmol/min/mg protein

Glucoamylase: ≥8.0 nmol/min/mg protein

Method Description

Disaccharidase activities are measured by incubating intestinal homogenates with an appropriate disaccharide substrate, which releases glucose. Glucose production is determined by a glucose oxidase reagent mixture that oxidizes glucose to produce peroxide, which reacts with phenol aminophenazone to form a red quinoneimine dye. The concentration of quinoneimine is determined spectrophotometrically, and the concentration of liberated glucose is calculated from a glucose standard curve. The amount of glucose produced is proportional to the disaccharidase activity in the intestinal homogenate. Disaccharidase activities are expressed as nanomoles of glucose per minute per milligram of protein (nmol/min/mg prot).(Unpublished Mayo method)

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82657

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DSAC Disaccharidase Activity Panel, Ts 57765-0

 

Result ID Test Result Name Result LOINC Value
608237 Lactase 81315-4
608238 Sucrase 30372-7
608239 Maltase 30315-6
608240 Palatinase 81314-7
608241 Glucoamylase 81322-0
608242 Interpretation 57779-1
608243 Reviewed By 18771-6