Test Code LAB4812 Galactose-Alpha-1,3-Galactose (Alpha-Gal), IgE, Serum
Additional Codes
SQ: FGA13M
Ordering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL for every 5 allergens requested
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
609737Useful For
As an aid in diagnosis of an IgE mediated hypersensitivity allergy to non-primate mammalian red meat, such as beef, pork, venison, and meat-derived products (eg, gelatin)
This test is not useful in patients previously treated with immunotherapy to determine if residual clinical sensitivity exists.
This test is not useful for patients in whom the medical management does not depend upon identification of allergen specificity.
Testing Algorithm
Other meat allergen IgE antibody tests may be considered in addition to galactose-alpha-1,3-galactose IgE antibody testing in cases of suspected red meat allergy.
Special Instructions
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Reporting Name
Galactose-alpha-1,3-galactose, IgESpecimen Type
SerumSpecimen Minimum Volume
For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 90 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Reference Values
Class |
IgE kU/L |
Interpretation |
0 |
<0.10 |
Negative |
0/1 |
0.10-0.34 |
Borderline/equivocal |
1 |
0.35-0.69 |
Equivocal |
2 |
0.70-3.49 |
Positive |
3 |
3.50-17.4 |
Positive |
4 |
17.5-49.9 |
Strongly positive |
5 |
50.0-99.9 |
Strongly positive |
6 |
≥100 |
Strongly positive |
Concentrations of 0.70 Ku/L or more (Class 2 and above) will flag as abnormally high
Reference values apply to all ages.
Method Description
Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away, and the bound complex incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Phadia AB; Rev 06/2020)
Day(s) Performed
Monday through Friday
Report Available
Same day/1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86008
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ALGAL | Galactose-alpha-1,3-galactose, IgE | 73837-7 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
ALGAL | Galactose-alpha-1,3-galactose, IgE | 73837-7 |
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Allergen Test Request (T236)
-Microbiology Test Request (T244)