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HelpTest Code LAB3977 Serotonin Release Assay, Unfractionated Heparin, Mass Spectrometry, Serum
Additional Codes
Mayo: SRAU
Sunquest: SRAUM
Shipping Instructions
Specimen should be frozen if shipped or stored for longer than 48 hours.
Specimen Required
Patient Preparation:
1. Specimen should be collected from a fasting (preferred, but not required) patient during an episode of suspected heparin-induced thrombocytopenia.
2. Patient should not be on ticagrelor (Brilinta) as this may interfere with the assay, yielding a false-negative result.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. After collection, specimen should sit at ambient temperature for a minimum of 1 hour in order to clot completely.
2. Centrifuge and aliquot serum into a plastic vial.
Specimen Stability Information: Frozen (preferred) 2 years/Refrigerate 7 days
Secondary ID
616216Useful For
Detecting heparin-dependent platelet activating antibodies implicated in the pathogenesis of heparin-induced thrombocytopenia
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Serotonin Release Assay, UFH, MS, SSpecimen Type
Serum RedSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Frozen (preferred) | ||
| Refrigerated | 7 days | ||
Reject Due To
| Gross lipemia | Reject |
Reference Values
Low Heparin Serotonin Release: <20%
High Heparin Serotonin Release: <20%
Serotonin Release Assay Result: Negative
Method Description
Patient serum is incubated with donor platelets and different concentrations of heparin. Anti-heparin platelet-factor 4/heparin antibodies present in the patient serum will bind to platelet Fc gamma receptors and activate donor platelets, releasing serotonin from the platelet granules. The released serotonin is measured utilizing liquid chromatography tandem mass spectrometry.(Carling RS, Degg TS, Allen KR, et al. Evaluation of whole blood serotonin and plasma and urine 5-hydroxyindole acetic acid in diagnosis of carcinoid disease. Ann Clin Biochem. 2002;39:577-582)
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| SRAU | Serotonin Release Assay, UFH, MS, S | 50736-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 616230 | Low Heparin Serotonin Release | 50728-5 |
| 616231 | High Heparin Serotonin Release | 50727-7 |
| 616232 | Serotonin Release Assay Result | 66488-8 |
| 616233 | Interpretation | 50733-5 |
| 616234 | Comment | 77202-0 |
| 616235 | Disclaimer | 62364-5 |
Forms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.