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Test Code LAB3810 Candida auris Surveillance, Molecular Detection, PCR, Varies

Additional Codes

SQ: CAURSM


Shipping Instructions


Specimen must arrive within 7 days of collection.



Necessary Information


Specimen source is required.



Specimen Required


Supplies: BD Eswab (T853)

Specimen Type: ESwab

Source: Axilla and groin composite

Container/Tube: ESwabs in liquid Amies medium

Specimen Volume: Swab

Collection Instructions:

1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media.

2. Swab used for this test cannot be shared with fungal culture. When fungal culture is ordered with this test, send separate swabs for each.


Secondary ID

607883

Useful For

Detecting Candida auris from surveillance swabs

 

This test should not be used to determine cure or to monitor response to therapy.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Candida auris Surveillance, PCR

Specimen Type

Swab

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Swab Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Wood shaft or charcoal swab
Clear semi-solid/solid media		 Reject
Culturette swab Reject
Clear semi-solid/solid media Reject

Reference Values

Not applicable

Method Description

Swabs are processed by placing 50 mcL of the Amies transport medium from an ESwab into neutralization buffer. DNA is extracted.

 

The extracted DNA is placed on the LightCycler 480 instrument, which amplifies and monitors by fluorescence the development of target nucleotide sequences after each polymerase chain reaction (PCR) cycle. A specific 269 base pairs target sequence from a portion of the 28S rDNA gene from Candida auris is amplified and the resulting segment is detected by melt-curve analysis using sequence-specific fluorescence resonance energy transfer hybridization probes. (Walchak RC, Buckwalter SP, Zinsmaster NM, et al. Candida auris direct detection from surveillance swabs, blood, and urine using a laboratory-developed PCR method. J Fungi (Basel). 2020;6(4):224. doi:10.3390/jof6040224)

Day(s) Performed

Monday through Sunday

Report Available

1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87481

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CAURS Candida auris Surveillance, PCR 95765-4

 

Result ID Test Result Name Result LOINC Value
SRCSR Specimen Source 31208-2
SCAR C auris PCR, Result 95765-4

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.