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HelpTest Code LAB3669 Vitamin B3 and Metabolites, Plasma
Additional Codes
SQ: VITB3M
MAYO: VITB3
Specimen Required
Patient Preparation: Fasting 4 to 8 hours preferred
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Lavender top (K2 EDTA)
Acceptable: K3 EDTA
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial within 2 hours of collection.
Secondary ID
604987Useful For
Assisting in the diagnosis of suspected vitamin B3 deficiency or toxicity
May be useful in determining response to therapy
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin B3 and Metabolites, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Nicotinic Acid (Niacin) Cutoff: <5.0 ng/mL
Nicotinamide: 5.0-48.0 ng/mL
Nicotinuric Acid Cutoff: <5.0 ng/mL
Method Description
Vitamin B3 components (nicotinic acid/niacin, nicotinamide, nicotinuric acid) are extracted from plasma specimens with internal standard and then analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84591
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VITB3 | Vitamin B3 and Metabolites, P | 102102-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 609493 | Nicotinic Acid (Niacin) | 18244-4 |
| 609494 | Nicotinamide | 56961-6 |
| 609495 | Nicotinuric Acid | 72306-4 |