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Test Code LAB324 Thrombin Time (Bovine), Plasma

Additional Codes

SQ: TTM

MAYO: TTSC


Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation: Fasting preferred

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot plasma into a separate plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally,  -40° C or below.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.

Secondary ID

602184

Useful For

Detecting or excluding the presence of heparin or heparin-like anticoagulants (which act by enhancing antithrombin's inhibition of thrombin and other procoagulant enzymes) when used in conjunction with the reptilase time (RT) in evaluating unexplained prolonged clotting times

 

Identifying the cause of a prolonged prothrombin time, activated partial thromboplastin time, or dilute Russell viper venom time when used in conjunction with the RT and fibrinogen assay

Method Name

Optical Clot-Based

Reporting Name

Thrombin Time (Bovine), P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Reference Values

15.8-24.9 seconds

Method Description

The thrombin time (TT) assay is performed on the Instrumentation Laboratory ACL TOP. Patient plasma is combined with a bovine thrombin reagent containing bovine albumin, calcium chloride, and buffer immediately triggering the coagulation process in the mixture. Time to clot formation is measured optically using a wavelength of 405 nm.(Package insert: HemosIL Thrombin Time, IL TOP Operators Manual. Instrumentation Laboratory Company; 06/2017)

Day(s) Performed

Monday through Saturday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

85670

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TTSC Thrombin Time (Bovine), P 46717-5

 

Result ID Test Result Name Result LOINC Value
TTSC Thrombin Time (Bovine), P 46717-5