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Test Code LAB2731 T-Cell Receptor Gene Rearrangement, PCR, Blood

Additional Codes

SQ: TCGRM

MAYO: TCGR

Reporting Name

T Cell Receptor Gene Rearrange, B

Useful For

Determining whether a T-cell population is polyclonal or monoclonal using blood specimens

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole blood


Shipping Instructions


Specimen must arrive within 7 days of collection.



Necessary Information


Include relevant clinical information and cytogenetic results, if available.



Specimen Required


Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred) 7 days
  Refrigerated  7 days

Reference Values

An interpretive report will be provided.

Positive, negative, or indeterminate for a clonal T-cell population

Day(s) Performed

Monday through Friday

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81340-TCB (T cell antigen receptor, beta) (eg, leukemia and lymphoma), gene rearrangement analysis to detect abnormal clonal population(s); using amplification methodology (eg, PCR)

81342-TCG (T cell receptor, gamma) (eg, leukemia and lymphoma), gene rearrangement analysis, evaluation to detect abnormal clonal population(s)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCGR T Cell Receptor Gene Rearrange, B In Process

 

Result ID Test Result Name Result LOINC Value
18210 Final Diagnosis: 22637-3
608951 Signing Pathologist 19139-5

Method Description

Genomic DNA is extracted from the blood. T-cell receptor beta (TCRB) and T-cell receptor gamma (TCRG) loci (official designations TRB and TRG, respectfully) are amplified by polymerase chain reaction (PCR) using a multiplex primer method based on the BIOMED-2 strategy. Specific primers are labeled with fluorochrome dyes, permitting precise fragment sizing of PCR products by capillary gel electrophoresis using a genetic analyzer. Each amplified locus is assessed for gene rearrangement patterns and an overall interpretation of the assay is made with regards to the presence or absence of a monoclonal population.(Unpublished Mayo method)

Report Available

5 to 10 days

Reject Due To

Gross hemolysis Reject
Moderately to severely clotted Reject

Method Name

Polymerase Chain Reaction (PCR)

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Secondary ID

83122