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HelpTest Code LAB259 Giardia Antigen, Feces
Additional Codes
SQ: GIARDM
MAYO: GIAR
Reporting Name
Giardia Ag, FUseful For
Sensitive screening for the detection of Giardia antigens present in fecal specimens
Testing Algorithm
The following algorithms are available:
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
FecalOrdering Guidance
Duodenal, colonic wash, or small bowel aspirates are not acceptable for this test. If giardiasis is suspected, order OPE / Ova and Parasite, Travel History or Immunocompromised, Feces.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Preserved feces
Supplies:
-Formalin 10% Buffered Neutral 15 mL (T466)
-Stool Collection Kit, Random (T635)
Container/Tube:
Preferred: Fecal container with 10% buffered formalin preservative
Acceptable: SAF (sodium acetate formalin)
Specimen Volume: 5 grams
Specimen Stability Information: Ambient (preferred) 60 days
Acceptable:
Specimen Type: Unpreserved feces
Supplies:
-Stool container, Small (Random), 4 oz (T288)
-Stool Collection Kit, Random (T635)
Container/Tube: Fecal container
Specimen Volume: 5 grams
Specimen Stability Information: Frozen 60 days
Specimen Minimum Volume
2 grams
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Fecal | Varies | ||
Special Instructions
Reference Values
Negative
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87329
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| GIAR | Giardia Ag, F | 6412-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 24085 | Giardia Ag, F | 6412-1 |
Method Description
Giardia antigens present in the stool supernatant are captured by antibodies coating the wells of a microtiter plate. The bound antigen is sandwiched by the addition of a second antibody, and the signal is amplified by adding biotin-streptavidin horseradish peroxidase. Blue color develops with the presence of bound antigen. The reaction is stopped with the addition of acid and read visually or with the aid of a spectrophotometer.(Rosenblatt JE, Sloan LM, Schneider SK. Evaluation of an enzyme-linked immunosorbent assay for the detection of Giardia lamblia in stool specimens. Diagn Microbiol Infect Dis. 1993;16(4):337-341; package insert: ProSpecT Giardia Microplate Assay. Oxoid; 03/2012)
Report Available
1 to 3 daysReject Due To
| Grossly bloody feces (containing no visible specimen) Very mucoid feces Specimens preserved in ECOFIX (green cap), C and S (orange cap), or methiolate formalin (MF) Duodenal aspirates Small bowel aspirates |
Reject |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)