Sign in →

Test Code LAB2534 Histoplasma Antibody Complement Fixation and Immunodiffusion, Serum

Additional Codes

SQ: HSTCRM

MAYO: HISER


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Secondary ID

621213

Useful For

Aiding in the diagnosis of active histoplasmosis

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Method Name

Complement Fixation (CF)/Immunodiffusion (ID)

Reporting Name

Histoplasma Ab CompFix/ImmDiff, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Anti-Yeast Antibody by Complement Fixation:

Negative (positive results reported as titer)

 

Antibody by Immunodiffusion:

Negative (positive results reported as band present)

Method Description

Both immunodiffusion and complement fixation (CF) tests are used to detect antibodies to Histoplasma capsulatum. For immunodiffusion, the antigen used is a culture filtrate. Histoplasmin H and M precipitins can be identified by the assay. For the CF test, antigens are histoplasmin and a yeast form antigen of Histoplasma capsulatum; the latter is more sensitive.(Roberts GD. Fungi. In: Washington II JA, ed. Laboratory Procedures in Clinical Microbiology. 2nd ed. Springer-Verlag, 1985; Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020)

Day(s) Performed

Monday through Friday

Report Available

2 to 7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86698 x2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HISER Histoplasma Ab CompFix/ImmDiff, S 90227-0

 

Result ID Test Result Name Result LOINC Value
621214 Histoplasma Yeast CompFix, S 20574-0
621215 Histoplasma Immunodiffusion, S 90232-0