Test Code LAB1928 Trichomonas vaginalis, Nucleic Acid Amplification, Varies
Additional Codes
SQ: TVRNAM
MAYO: TVRNA
Useful For
Detecting Trichomonas vaginalis in urine, cervical/endocervical or vaginal specimen types
Reporting Name
Trichomonas vaginalis Amplified RNASpecimen Type
VariesNecessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Endocervix/Cervix
Supplies: Aptima Unisex Swab Collection Kit (T583)
Container/Tube: Aptima Unisex Swab
Specimen Volume: Swab
Collection Instructions:
1. Specimens must be collected using the Aptima Unisex Swab Collection Kit.
2. Use cleaning swab (white shaft) to remove excess mucus from endocervix/cervix.
Discard the cleaning swab.
3. Insert second swab (blue shaft) 1 to 1.5 cm into endocervical canal, and rotate swab gently for 30 seconds. Avoid touching vaginal wall when removing swab.
4. Place second swab (blue shaft) into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
5. Cap tube securely and label tube with patient's entire name and collection date and time.
6. Maintain swab container at 2 to 30° C (refrigerate temperature is preferred), transport within 60 days of collection.
Specimen Type: Vaginal
Supplies: Aptima Multitest Swab Collection Kit (T584)
Container/Tube: Aptima Multitest Swab
Specimen Volume: Swab
Collection Instructions:
1. Specimens must be collected using the Aptima Multitest Swab Collection Kit.
2. Insert swab (pink shaft) about 5 cm past introitus and rotate gently for 30 seconds.
3. Place swab into Aptima transport tube provided in collection kit. Snap off swab at score line so swab fits into closed tube.
4. Cap tube securely and label tube with patient's entire name and collection date and time.
5. Maintain swab container at 2 to 30° C (refrigerate temperature is preferred), transport within 60 days of collection.
Specimen Type: ThinPrep Specimen (Endocervix/Cervix)
Supplies: Aptima Thin Prep Transport Tube (T652)
Container/Tube: Thin Prep Transport Tube
Specimen Volume: 1 mL
Collection Instructions:
1. Collect ThinPrep sample as per normal collection process.
2. ThinPrep specimen must be aliquoted (as outlined below) before it is processed/tested for Pap smear.
3. Vortex ThinPrep/PreservCyt vial 3 to 10 seconds. Within 1 minute of vortexing:
a. Transfer 1 mL of specimen into the Aptima specimen transfer tube using a disposable transfer pipette or a pipette tip containing a filter (aerosol barrier or hydrophobic plug).
b. Process only 1 ThinPrep and transfer tube set at a time.
c. Recap Aptima specimen transfer tube tightly and gently invert 3 times to mix.
3. Label Aptima transfer tube with appropriate label.
4. Use remainder of ThinPrep specimen for Pap testing.
5. Maintain specimen transport tube at 2 to 30° C (refrigerate temperature is preferred), transport within 30 days of collection when stored at 2 to 14° C or within 14 days when stored at 15 to 30° C.
Specimen Type: Urine
Supplies: Aptima Urine Transport Tube (T582)
Container/Tube: Aptima urine specimen transport tube
Specimen Volume: 15 to 20 mL
Collection Instructions:
1. Patient should not have urinated for at least 1 hour prior to specimen collection.
2. Patient should collect first portion of random voided urine (first part of stream) into a sterile, plastic, preservative-free container.
3. Within 24 hours of collection, transfer 2 mL of urine into the Aptima urine specimen transport tube using the disposable pipette provided. The correct volume of urine has been added when the fluid level is between the black fill lines on the Aptima urine transport tube.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Refrigerated (preferred) | APTIMA VIAL | |
Frozen | 180 days | APTIMA VIAL | |
Ambient | APTIMA VIAL |
Reject Due To
Midstream urine specimen Clean catch urine specimen Over-filled or under-filled urine transport tubes Specimen collected into a SurePath device Transport tubes containing a cleaning swab or more than 1 swab |
Reject |
Reference Values
Negative
Method Description
The APTIMA Trichomonas vaginalis assay combines the technologies of target capture, transcription-mediated amplification, and hybridization protection assay for detection of 16S ribosomal RNA from T vaginalis.(Package insert: APTIMA Trichomonas vaginalis Assay. Hologic, Inc; Rev. 003, 06/2023)
Day(s) Performed
Monday through Sunday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87661
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TVRNA | Trichomonas vaginalis Amplified RNA | 46154-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
SRC29 | SOURCE: | 31208-2 |
34810 | Trichomonas vaginalis amplified RNA | 46154-1 |
Method Name
Transcription-Mediated Amplification
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.