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Test Code Epic LAB801 Measles (Rubeola) Antibodies, IgM, Serum

Additional Codes

SQ code ROMM
Mayo code ROM

Reporting Name

Measles (Rubeola) Ab, IgM, S

Specimen Type

Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Determining acute-phase infection with rubeola (measles) virus using IgM antibody testing

 

Aiding in the identification of nonimmune individuals through IgM antibody testing


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86765

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ROM Measles (Rubeola) Ab, IgM, S 35276-5

 

Result ID Test Result Name Result LOINC Value
80979 Measles (Rubeola) Ab, IgM, S 35276-5

Reject Due To

Gross hemolysis Gross reject
Gross lipemia Gross reject
Heat-inactivated specimen Reject

Method Name

Immunofluorescence Assay (IFA)

Method Description

The presence of IgM-class antibody to measles is determined by an indirect immunofluorescence assay. After removal of IgG by specific immunoglobulin antibody, the serum is incubated with measles antigen, which is adhered to a glass microscope slide. Antibodies, if present, will bind to the antigen forming stable antigen-antibody complexes. If no antibodies are present, the complexes will not be formed, and the serum components will be washed away. Fluorescein-labeled antihuman-IgM antibody is added to the reaction side and binds to IgM antibodies if present. This results in a positive reaction of bright apple-green fluorescence when viewed with a fluorescence microscope.(Package insert: Measles Virus Antigen Substrate Slide. AESKU.BION; 09/2019)

Report Available

Same day/1 to 3 days

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.