Test Code Epic LAB657 Measles (Rubeola) Antibodies, IgG, Serum
Additional Codes
SQ code RBIGGM
Mayo code ROPG
Reporting Name
Measles (Rubeola) Ab, IgG, SSpecimen Type
SerumPerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Determining immune status of individuals to the measles virus
Documenting previous infection with measles virus in an individual without a previous record of immunization to measles virus
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Instructions: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 14 days |
Reference Values
Vaccinated: positive (≥1.1 AI)
Unvaccinated: negative (≤0.8 AI)
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86765
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ROPG | Measles (Rubeola) Ab, IgG, S | 77310-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
ROG | Measles (Rubeola) Ab, IgG, S | 35275-7 |
DEXG3 | Measles IgG Antibody Index | 5244-9 |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Heat-inactivated specimen | Reject |
Method Name
Multiplex Flow Immunoassay (MFI)
Method Description
The BioPlex 2200 Measles IgG assay uses multiplex flow immunoassay technology. Briefly, serum samples are mixed and incubated at 37° C with sample diluent and dyed beads coated with measles antigen. After a wash cycle, antihuman-IgG antibody conjugated to phycoerythrin (PE) is added to the mixture and incubated at 37° C. Excess conjugate is removed in another wash cycle and the beads are resuspended in wash buffer. The bead mixture then passes through a detector that identifies the bead based on dye fluorescence and determines the amount of antibody captured by the antigen based on the fluorescence of the attached PE. Raw data is calculated in relative fluorescence intensity.
Three additional dyed beads, an internal standard bead, a serum verification bead, and a reagent blank bead are present in each reaction mixture to verify detector response, the addition of serum to the reaction vessel, and the absence of significant nonspecific binding in serum.(Package insert: BioPlex 2200 System MMRV IgG, Bio-Rad Laboratories; 02/2019)
Report Available
Same day/1 to 3 daysForms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.