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Test Code Epic LAB571 Glucose 6-Phosphate Dehydrogenase Enzyme Activity, Blood

Additional Codes

SQ: G6PDM

MAYO: G6PD1


Specimen Required


Collection Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: Lavender top (EDTA) or yellow top (ACD solution A)

Specimen Volume: 6 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.


Secondary ID

607460

Useful For

Evaluation of individuals with episodic or chronic Coombs-negative nonspherocytic hemolytic anemia

 

Rapid testing to assess glucose 6-phosphate dehydrogenase (G6PD) enzyme capacity prior to rasburicase or other therapies that may cause hemolysis or methemoglobinemia in G6PD deficient patients

 

May aid in the creation of a comprehensive patient profile and can ensure appropriate patient monitoring for developing anemia

Method Name

Kinetic Spectrophotometry

Reporting Name

G6PD Enzyme Activity, B

Specimen Type

Whole Blood ACD-B

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 20 days

Reject Due To

Gross hemolysis Reject

Reference Values

≥12 months of age: 8.0-11.9 U/g Hb

 

Reference values have not been established for patients who are less than 12 months of age.

Method Description

Glucose 6-phosphate dehydrogenase in a hemolysate catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconate. Concomitantly, nicotinamide adenine dinucleotide phosphate (NADP[+]) is changed to its reduced form, NADPH, and the reaction is measured spectrophotometrically on an automated chemistry analyzer.(Beutler E. Red cell metabolism: A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:68-71; van Solinge WW, van Wijk. Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82955

LOINC Code Information

Test ID Test Order Name Order LOINC Value
G6PD1 G6PD Enzyme Activity, B 32546-4

 

Result ID Test Result Name Result LOINC Value
G6PCL G6PD Enzyme Activity, B 32546-4

Day(s) Performed

Monday through Friday

Report Available

1 to 4 days

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.