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Test Code Epic LAB5365 Human T-Cell Lymphotropic Virus Types I and II (HTLV-I/-II) Antibody Confirmation, Serum

Additional Codes

SQ code HTLVLM

Mayo code HTLVL

Reporting Name

HTLV-I/-II Ab Confirmation, S

Specimen Type

Serum SST

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Confirmatory detection of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II)-specific IgG antibodies in human serum specimens that are consistently reactive by initial screening tests

 

Differentiating between HTLV-I- and HTLV-II-specific IgG antibodies


Ordering Guidance


This confirmatory assay should be ordered only on serum specimens that are consistently reactive by an antihuman T-cell lymphotropic virus 1 and 2 (anti-HTLV-I/-II) screening immunoassay. For an evaluation that includes screening and confirmation, order HTLVI / Human T-Cell Lymphotropic Virus Types I and II Antibody Screen with Confirmation, Serum.

 

For testing spinal fluid specimens, order HTLLC / Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Confirmation, Spinal Fluid.



Necessary Information


Date of collection is required.



Specimen Required


Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.


Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred)
  Refrigerated  7 days

Reference Values

Negative

Day(s) Performed

Tuesday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86689

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HTLVL HTLV-I/-II Ab Confirmation, S 55162-2

 

Result ID Test Result Name Result LOINC Value
83277 HTLV-I/-II Ab Confirmation, S 22362-8
23898 HTLV-I/-II Bands 61112-9
23899 HTLV-I/-II Discrimination 77744-1

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Line Immunoassay (LIA)

Method Description

INNO-LIA HTLV I/II Score is a line immunoassay based on the enzyme immunoassay principle. The assay uses well-defined antigens derived from human T-cell lymphotropic virus types 1 and 2 (HTLV-1 and HTLV-2) immunodominant proteins. The antigens used are either recombinant proteins or synthetic peptides, highly purified and fixed on a nylon membrane strip. The sequences are selected to allow the detection of antibodies with a wide specificity to all known isolates of the HTLV strains. The antigenicity exhibited by these proteins and peptides is either common to both HTLV-1 and HTLV-2, or type-specific to 1 of the 2 viruses to allow confirmation and discrimination in a single assay. Two gag (p19-I/II, p24-I/II) and 2 env (gp46-I/II, gp21-I/II) bands are applied as non-type-specific antigens, which are used to confirm the presence of antibodies against HTLV-1/2. The type-specific antigens for HTLV-1 (gag p19-I, env gp46-I) and for HTLV-2 (env gp46-II) are applied to differentiate between HTLV-1 and HTLV-2 infections. In addition, 4 control lines are coated: 1 negative control (streptavidin), and 3 positive control lines: a strong (antihuman IgG), a moderate (human IgG), and a weak (human IgG) line.

 

A test sample is incubated in a test trough together with the multiple antigen-coated strip. Specific HTLV antibodies, if present in the sample, will bind to the HTLV antigen lines on the strip. Subsequently, goat-antihuman IgG labeled with alkaline phosphatase is added and will bind to any HTLV antigen-antibody complex previously formed. Incubation with a chromogenic substrate produces a dark brown color in proportion to the amount of specific antibodies present in the sample. The color development is stopped with sulfuric acid. If the sample contains no HTLV-specific antibodies, only a low background color develops.(Unpublished Mayo method)

Report Available

2 to 15 days

Secondary ID

83277

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.