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Test Code Epic LAB492 Protein S Antigen, Plasma

Additional Codes

SQ code PTSAFM

Mayo code PSTF

Reporting Name

Protein S Ag, P

Specimen Type

Plasma Na Cit

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Investigation of patients with a history of thrombosis

Testing Algorithm

If this test result is decreased, then total plasma protein S antigen will be performed at an additional charge.


Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation: Patient must not be receiving heparin or Coumadin. If the patient is being treated with Coumadin, this should be noted. Coumadin will lower protein S.

 

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 1 mL in 2 plastic vials each containing 0.5 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot 0.5 mL of plasma into 2 plastic vials, leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally  -40° C or below.

 

5. Send specimens in the same shipping container.

Additional Information: A double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reference Values

TOTAL

Males: 80-160%

Females

<50 years: 70-160%

≥50 years: 80-160%

 

FREE

Males: 65-160%

Females

<50 years: 50-160%

≥50 years: 65-160%

 

Normal, full-term newborn infants or healthy premature infants may have decreased levels of total protein S (15-50%); but because of low levels of C4b-binding protein, free protein S may be normal or near the normal adult level (≥50%). Total protein S reaches adult levels by 90 to 180 days postnatal.*

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing

Day(s) Performed

Monday through Friday

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85306-Free

85305-Total (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PSTF Protein S Ag, P 87557-5

 

Result ID Test Result Name Result LOINC Value
PSF Protein S Ag, Free, P 27821-8

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

PSF, PST: Latex Immunoassay (LIA)

Profile Information

Test ID Reporting Name Available Separately Always Performed
PSF Protein S Ag, Free, P No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
PST Protein S Ag, Total, P No No

Method Description

Total:

This assay is performed using the Diagnostica Stago LIATEST Protein S Kit on the Beckman Coulter ACL TOP. Protein S total antigen is determined using automated latex immunoassay methodology. This methodology is comprised of a reagent with microlatex particles coated with specific antihuman total protein S antibodies. Patient plasma containing total protein S antigen is combined with the latex reagent causing the antibody-coated latex particles to agglutinate and form aggregates. The aggregates form diameters greater than the wavelength of the light (405 nm) passing through causing absorption of the light. This change in absorption is measured over time and reported as delta optical density (OD). The increase in absorption is proportional to the concentration of protein S total antigen present in the patient plasma.(Package insert: Liatest Protein S, Diagnostica Stago; 11/2015)

 

Free:

This assay is performed using the HemosIL Free Protein S kit on the Beckman Coulter ACL TOP. The assay uses latex immunoassay methodology to determine the presence of free protein S. It consists of 2 latex reagents, one being latex particles coated with purified human C4BP and the other is latex particles coated with a monoclonal antibody directed against human protein S. Patient plasma is combined with the purified C4BP which reacts with a high affinity for free protein S in the patient plasma. The free protein S adsorbed on the C4BP latex triggers the agglutination reaction with the second latex reagent. The aggregates form diameters greater than the wavelength of the light (405 nm) passing through causing absorption of the light. This change in absorption is measured over time and reported as delta OD. The increase in absorption is proportional to the concentration of free protein S antigen present in the patient plasma.(Package insert: HemosIL Free Protein S. Instrumentation Laboratory Company; 04/2019)

Report Available

1 to 3 days

Secondary ID

83049