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HelpTest Code Epic LAB468 Diazepam and Metabolites, Serum
Additional Codes
SQ code DIAZPM
Mayo code DIA
Test Down Notes
This test is temporarily unavailable. As an alternate, order ZW86(1501SP). For additional details, see test update here.
Reporting Name
Diazepam and metabolites, SSpecimen Type
Serum RedPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Assessing compliance
Monitoring for appropriate therapeutic level
Assessing diazepam toxicity
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reference Values
Therapeutic concentrations
Diazepam: Not established
Diazepam and Nordiazepam: 100-2,500 ng/mL
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Diazepam: 10 ng/mL
Nordiazepam: 10 ng/mL
Oxazepam: 10 ng/mL
Temazepam: 10 ng/mL
Day(s) Performed
Wednesday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80299
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DIA | Diazepam and metabolites, S | 49044-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 8629 | Diazepam | 3548-5 |
| 2475 | Nordiazepam | 3537-8 |
| 2459 | Diazepam and Nordiazepam | 16757-7 |
| 622868 | Oxazepam | 3886-9 |
| 622869 | Temazepam | 10343-2 |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
The internal standard mixture containing chlordiazepoxide-d5, diazepam-d5 , nordiazepam-d5, oxazepam-d5, and temazepam-d5 is added to serum samples, mixed, and centrifuged. The supernatant is diluted and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)
Report Available
3 to 7 daysForms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.