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Test Code Epic LAB468 Diazepam and Nordiazepam, Serum

Additional Codes

SQ code DIAZPM

Mayo code DIA

Test Down Notes

Effective December 14, 2023:  This test is temporarily unavailable due to analytic issues.  The downtime is expected to be >30 days.  Order ZW86 (1501SP) as an alternate.  See test notification here.

Reporting Name

Diazepam and Nordiazepam, S

Specimen Type

Serum Red

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Assessing compliance

 

Monitoring for appropriate therapeutic level

 

Assessing diazepam toxicity


Specimen Required


Collection Container/Tube: Red top (Serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 14 days
  Ambient  14 days
  Frozen  14 days

Reference Values

Therapeutic concentrations

Diazepam and Nordiazepam: 200-2,500 ng/mL

Day(s) Performed

Monday, Wednesday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80299

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DIA Diazepam and Nordiazepam, S 49044-1

 

Result ID Test Result Name Result LOINC Value
8629 Diazepam 3548-5
2475 Nordiazepam 3537-8
2459 Diazepam and Nordiazepam 16757-7

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Method Description

The internal standard mixture containing chlordiazepoxide-d5, diazepam-d4, and nordiazepam-d5 is added to serum samples. The serum samples are treated with phosphate buffer and extracted via liquid/liquid extraction. The organic layer from the extraction is dried under nitrogen, reconstituted, and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)

Report Available

3 to 7 days

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Secondary ID

8629