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Test Code Epic LAB4148 Coenzyme Q10, Total, Plasma

Additional Codes

SQ code TQ10M

Mayo TQ10

Reporting Name

Coenzyme Q10, Total, P

Specimen Type

Plasma Heparin

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnosis of primary coenzyme Q10 (CoQ10) deficiencies in some patients who are not supplemented with CoQ10

 

Monitoring patients receiving statin therapy

 

Monitoring CoQ10 status during treatment of various degenerative conditions, including Parkinson and Alzheimer diseases

 

Providing accurate quantitation of total CoQ10 when specimens are hemolyzed

 

This test is not useful for distinguishing primary CoQ10 deficiencies from acquired CoQ10 deficiencies.


Ordering Guidance


This test does not quantitate reduced coenzyme Q10 (CoQ10). For reduced CoQ10, order Q10 / Coenzyme Q10, Reduced and Total, Plasma.

 

The most reliable test for the diagnosis of primary defects in ubiquinone (ie, CoQ10) biosynthesis is direct measurement of CoQ10 in muscle.



Necessary Information


Patient's age is required.



Specimen Required


Patient Preparation:

Fasting: 8 hours, required

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Green top (lithium or sodium heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL plasma

Collection Instructions:

1. Immediately after collection, place specimen on wet ice. Maintain on wet ice until processing.

2. Within 3 hours of collection, centrifuge, aliquot plasma into a plastic vial, and freeze immediately.


Specimen Minimum Volume

Plasma: 0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Heparin Frozen (preferred) 60 days
  Refrigerated  7 days
  Ambient  4 days

Reference Values

<18 years: 320-1558 mcg/L

≥18 years: 433-1532 mcg/L

 

Miles MV, Horn PS, Tang PH, et al. Age-related changes in plasma coenzyme Q10 concentrations and redox state in apparently healthy children and adults. Clin Chim Acta. 2004;347(1-2):139-144

Day(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

82542

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TQ10 Coenzyme Q10, Total, P 27923-2

 

Result ID Test Result Name Result LOINC Value
36764 CoQ10 Total 27923-2
36765 Interpretation (TQ10) 59462-2
36777 Reviewed By 18771-6

Reject Due To

Gross hemolysis OK
Gross lipemia Reject
Gross icterus OK

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Genetics Test Information

This test is used for the diagnosis of coenzyme Q10 (CoQ10) deficiency in mitochondrial disorders. It is also used to monitor CoQ10 status in patients with mitochondrial cytopathies, patients receiving statin therapy, or during treatment of various degenerative conditions including Parkinson and Alzheimer diseases.

Method Description

Coenzyme Q10, together with other lipid soluble substances, is extracted from plasma with cold n-propanol containing coenzyme Q10-d9 as an internal standard. An aliquot of the lipid extract is analyzed by liquid chromatography tandem mass spectrometry. The isolated coenzymeQ10 peaks corresponding to the reduced form (CoQ10H2) and the oxidized form CoQ10 are quantified by measurement off extracted and analyzed calibration curves for the respective form of Q10. Chromatography is performed using a C18 (30x2.1mm) column and total analysis time is 3.5 minutes.(Unpublished Mayo method)

Report Available

2 to 4 days

Secondary ID

63148