Test Code Epic LAB3971 Nicotine and Metabolites, Random, Urine
Additional Codes
SQ code NICOUM
Mayo code NICOU
Reporting Name
Nicotine and Metabolites, USpecimen Type
UrinePerforming Laboratory
Mayo Clinic Laboratories in RochesterUseful For
Monitoring tobacco use
Monitoring patients on nicotine-replacement therapy for concurrent use of tobacco products
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plastic, 5 mL, aliquot tube
Specimen Volume: 3 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Specimen Minimum Volume
2 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 28 days | |
Frozen | 365 days | ||
Ambient | 28 days |
Reference Values
Non-tobacco user with no passive exposure:
NICOTINE
<5.0 ng/mL
COTININE
<5.0 ng/mL
ANABASINE
<2.0 ng/mL
NORNICOTINE
<2.0 ng/mL
Day(s) Performed
Monday through Thursday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80323
G0480 (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
NICOU | Nicotine and Metabolites, U | 43125-4 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
82510 | Nicotine | 3854-7 |
21321 | Cotinine | 10366-3 |
21324 | Nornicotine | 33917-6 |
21323 | Anabasine | 33915-0 |
Reject Due To
Gross hemolysis | OK |
Gross icterus | OK |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Method Description
Nicotine and metabolites are extracted from urine by solid-phase extraction techniques. The extract eluate is quantified by high-performance liquid chromatography tandem mass spectrometry.(Moyer TP, Charlson JR, Enger RJ, et al. Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002;48[9]:1460-1471; Oh J, Park MS, Chun MR, et al. A simple and high-throughput LC-MS/MS method for simultaneous measurement of nicotine, cotinine, 3-OH cotinine, nornicotine, and anabasine in urine and its application in the general Korean population. J Anal Toxicol. 2022:46[1]:25-36. doi:10.1093/jat/bkaa177)
Report Available
2 to 5 daysForms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.