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Test Code Epic LAB3798 Hepatitis E Virus IgM Antibody Screen with Reflex to Confirmation, Serum

Additional Codes

SQ code HEVMM

Mayo code HEVM

Reporting Name

HEV IgM Ab Screen, S

Specimen Type

Serum SST

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnosis of acute or recent (<6 months) hepatitis E infection

Testing Algorithm

If hepatitis E virus (HEV) IgM antibody screen is reactive or borderline, HEV IgM antibody confirmation will be performed at an additional charge.

 

For more information see Hepatitis E: Testing Algorithm for Diagnosis and Management.


Necessary Information


Date of collection is required.



Specimen Required


Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).

2. Aliquot serum into plastic vial.


Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred)
  Refrigerated  24 hours

Reference Values

Negative

Day(s) Performed

Tuesday, Thursday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86790

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HEVM HEV IgM Ab Screen, S 14212-5

 

Result ID Test Result Name Result LOINC Value
86212 HEV IgM Ab Screen, S 14212-5

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Enzyme Immunoassay (EIA)

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HEVML HEV IgM Ab Confirmation, S Yes No

Method Description

This is a qualitative, in vitro test for the detection and identification of IgM antibodies specifically against hepatitis E virus (HEV) in human serum. This assay is a screening test based on the principle of an indirect sandwich enzyme-linked immunosorbent assay.

 

Highly purified recombinant HEV-ORF2 viral antigens (specific for HEV genotypes 1 and 3) are fixed to microplate wells. Diluted patient serum specimens are incubated in the wells, in which antibodies bind specifically to the HEV recombinant antigens coating the surface of the wells. Unbound antibodies are then washed away. Anti-human immunoglobulin antibodies (IgM), which are coupled to horseradish peroxidase, are then added to the wells and incubated. Unbound conjugate antibodies are then washed away. Specifically, bound antibodies are detected by a peroxidase-catalyzed color reaction. Intensity of the color, as measured with a photometric analyzer, is proportionate to the quantity of bound HEV IgM antibodies present in the serum specimen.(Unpublished Mayo method)

Report Available

1 to 7 days

Forms

If not ordering electronically, complete, print, and send 1 of the following:

-Gastroenterology and Hepatology Test Request (T728)

-Infectious Disease Serology Test Request (T916)

-Microbiology Test Request (T244)