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HelpTest Code Epic LAB3507 Liver/Kidney Microsome Type 1 Antibodies, Serum
Additional Codes
SQ code LKMAB
Mayo code LKM
Reporting Name
Liver/Kidney Microsome Type 1 Ab, SSpecimen Type
SerumPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of patients with liver disease of unknown etiology
Evaluation of patients with suspected autoimmune hepatitis
Testing Algorithm
For more information see First-Line Screening for Autoimmune Liver Disease Algorithm.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Special Instructions
Reference Values
≤20.0 Units (Negative)
20.1-24.9 Units (Equivocal)
≥25.0 Units (Positive)
Reference values apply to all ages.
Day(s) Performed
Monday, Wednesday, Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86376
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LKM | Liver/Kidney Microsome Type 1 Ab, S | 32220-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LKM | Liver/Kidney Microsome Type 1 Ab, S | 32220-6 |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat treated | Reject |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
Purified full-length recombinant human cytochrome P450 2D6 antigen is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Pre-diluted controls and diluted patient sera are added to separate wells, allowing any liver/kidney microsomal antibodies type 1 present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme labeled anti-human IgG antibody (conjugate) is added to each well. A second incubation allows the enzyme labeled anti-human IgG antibody to bind any patient antibodies, which have become attached to the microwells. After washing away any unbound enzyme labeled anti-human IgG antibody, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. The assay is evaluated by spectrophotometrically measuring and comparing the color intensity that develops in the patient wells with the color in the calibrator wells. (Package insert: INOVA Diagnostics, Inc.; Revision 13; 10/2018)
Report Available
2 to 4 daysForms
If not ordering electronically, complete, print, and send 1 of the following with the specimen:
-Gastroenterology and Hepatology Test Request (T728)
-General Request (T239)