Sign in →

Test Code Epic LAB338 Syphilis Antibody, Treponema pallidum Particle Agglutination, Serum

Additional Codes

SQ code TPPAM

Mayo code TPPA

Reporting Name

Syphilis Ab by TP-PA, S

Specimen Type

Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

An aid to resolve discrepant results between screening treponemal and non-treponemal assays

 

This test is not recommended for general screening purposes for syphilis.

 

This test should not be used to evaluate response to therapy.

 

This test is not intended for medical-legal use.

Testing Algorithm

For more information see Syphilis Serology Algorithm.


Ordering Guidance


This assay is recommended by the Centers for Disease Control and Prevention for sera testing positive by a screening treponemal assay and negative by rapid plasma reagin (RPR). The results of this assay assist in determining whether the results of a screening treponemal test are truly or falsely positive.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Special Instructions

Reference Values

Negative

Day(s) Performed

Monday through Friday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86780

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TPPA Syphilis Ab by TP-PA, S 24312-1

 

Result ID Test Result Name Result LOINC Value
61480 Syphilis Ab by TP-PA, S 24312-1

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Particle Agglutination

Method Description

The Serodia Treponema pallidum particle agglutination (TP-PA) test is based on the agglutination of colored gelatin particle carriers sensitized with Treponema pallidum (Nichols Strain) antigen. Serum samples are serially diluted in microplate wells. Sensitized gelatin particles are added to respective wells and the contents of the plate mixed. The mixture is incubated for 2 hours at ambient temperature. Serum containing specific antibodies will react with the antigen-sensitized colored gelatin particles to form a smooth mat of agglutinated particles in the microplate well. A compact button formed by the settling of the non-agglutinated particles characterizes negative reactions. The agglutination patterns are read visually to determine interpretation.(Package insert: Serodia TP-PA. Fujirebio Diagnostics, Inc; 08/2017)

Report Available

Same day/1 to 4 days

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.