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Test Code Epic LAB2819 Pyridoxal 5-Phosphate, Plasma

Additional Codes

SQ code PLPM

Mayo code PLP

Reporting Name

Pyridoxal 5-Phosphate (PLP), P

Specimen Type

Plasma Heparin

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Determining vitamin B6 status, including in persons who present with progressive nerve compression disorders, such as carpal tunnel and tarsal tunnel syndromes

 

Determining the overall success of a vitamin B6 supplementation program

 

Diagnosis and evaluation of hypophosphatasia


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation:

1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.

2. For 24 hours before specimen collection, patient should not take multivitamins or vitamin supplements.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Green top (sodium or lithium heparin) or Plasma gel separator (PST)

Acceptable: None

Submission Container/Tube: Amber vial

Specimen Volume: 1 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge at 4° C.

2. Aliquot all plasma into amber vial and freeze immediately.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Frozen 29 days LIGHT PROTECTED

Reference Values

5-50 mcg/L

Day(s) Performed

Monday through Thursday, Saturday, Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84207

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLP Pyridoxal 5-Phosphate (PLP), P 30552-4

 

Result ID Test Result Name Result LOINC Value
4047 Pyridoxal 5-Phosphate (PLP), P 30552-4

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Secondary ID

42359

Report Available

2 to 7 days

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.

Method Description

The stable isotope pyridoxal 5-phosphate-d2 and/or pyridoxic acid-d2 is added to plasma as an internal standard. Meta-phosphoric acid solution is then added to precipitate the proteins. Following sedimentation of the proteins, an aliquot of the clarified supernatant fluid is subjected to separation of pyridoxal 5-phosphate, pyridoxic acid, and internal standards from other plasma components by reverse-phase high-performance liquid chromatography with quantitation by tandem mass spectrometry.(Maus A, Girtman A, Kiesling J, Faber J, Grebe SKG. Overcoming the chromatographic challenges when performing LC-MS/MS measurements of pyridoxal-5'-phosphate. J Chromatogr B Analyt Technol Biomed Life Sci. 2023;1217:123605. doi:10.1016/j.jchromb.2023)