Method Description

IgM:

The ASI Mumps IgM EIA (enzyme immunoassay) is based on the principle of the capture of serum immunoglobulins by anti-human IgM monoclonal antibodies found on the solid phase. A subsequent incubation with mumps antigen in a complex with monoclonal antibodies conjugated to horse radish peroxidase selects the IgM antibodies specific for the antigen. Peroxidase substrate is added and the enzymatic reaction is stopped by the addition of a sulfuric acid solution which results in a yellow color change. The intensity of the yellow color is proportional to the amount of specific antibodies present in the sample and is read in an enzyme-linked immunosorbent assay microplate reader.(Package insert: ASI Mumps IgM EIA test. Arlington Scientific Inc; 860096AM Rev 03/14/2025)

IgG:

The BioPlex 2200 Mumps IgG assay uses multiplex flow immunoassay technology. Briefly, serum samples are mixed and incubated at 37° C with sample diluent and dyed beads coated with mumps antigen. After a wash cycle, antihuman-IgG antibody conjugated to phycoerythrin (PE) is added to the mixture and incubated at 37° C. Excess conjugate is removed in another wash cycle, and the beads are resuspended in wash buffer. The bead mixture then passes through a detector that identifies the bead based on dye fluorescence and determines the amount of antibody captured by the antigen by the fluorescence of the attached PE. Raw data is calculated in relative fluorescence intensity. Three additional dyed beads, an internal standard bead, a serum verification bead, and a reagent blank bead are present in each reaction mixture to verify detector response, the addition of serum to the reaction vessel, and the absence of significant nonspecific binding in serum.(Package insert: BioPlex 2200 System MMRV IgG. Bio-Rad Laboratories; 02/2019)