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HelpTest Code Epic LAB2548 Human T-Cell Lymphotropic Virus Types I and II Antibody Screen with Confirmation, Serum
Additional Codes
SQ code HTLVIM
Mayo code HTLVI
Reporting Name
HTLV-I/-II Ab Screen, SSpecimen Type
SerumPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Qualitative detection of human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II)-specific antibodies with confirmation and differentiation between HTLV-I and HTLV-II infection
This test should not be used to screen blood, human cells, tissues, or solid-organ donors.
This test is not intended for use on cord blood specimens.
Testing Algorithm
If the human T-cell lymphotropic virus types I and II (HTLV-I/-II) antibody screen is reactive, then HTLV-I/-II antibody confirmation by line immunoassay will be performed at an additional charge.
Ordering Guidance
This test is for serum specimens only. For spinal fluid specimens, order HTLVC / Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Screen with Confirmation, Spinal Fluid.
Necessary Information
Date of collection is required.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge blood collection tube per collection tube manufacturer's instructions (eg, centrifuge within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot serum into plastic vial.
Specimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 28 days | |
| Refrigerated | 7 days |
Reference Values
Negative
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86790
86689 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HTLVI | HTLV-I/-II Ab Screen, S | 29901-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 9539 | HTLV-I/-II Ab Screen, S | 29901-6 |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat-treated or precipitated specimens | Reject |
Method Name
Enzyme Immunoassay (EIA)
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HTLVL | HTLV-I/-II Ab Confirmation, S | Yes | No |
Method Description
The Avioq HTLV-I/II Microelisa System is an enzyme-linked immunosorbent assay in which the solid phase (microwells) is coated with a purified human T-cell lymphotropic virus types I (HTLV-I) viral lysate, a purified HTLV-II viral lysate, and a recombinant HTLV-I p21E antigen. With the addition of a diluted test sample containing antibodies to either HTLV-I or HTLV-II, complexes are formed by the interaction of the antibodies in the sample and the solid phase antigens. Following incubation, the sample is aspirated and the well is washed with buffer. Subsequently, antihuman immunoglobulin (goat) conjugated with horseradish peroxidase (HRP) is added, which binds the antibody-antigen complex during a second incubation. Following a wash and incubation with TMB (tetramethylbenzidine) substrate, a blue color is produced. The enzyme reaction is stopped by the addition of a sulfuric acid solution, which changes the color to yellow. The amount of HTLV-I / HTLV-II specific antibodies present in the sample is proportional to the color intensity.(Package insert: Avioq HTLV-I/II Microelisa System. Avioq, Inc; 05/2019)
Report Available
1 to 4 daysSecondary ID
9539Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.