Home
HelpTest Code Epic LAB181 Beta-2-Microglobulin (Beta-2-M), Spinal Fluid
Additional Codes
SQ code SFB2M
Mayo code B2MC
Reporting Name
Beta-2-Microglobulin, CSFSpecimen Type
CSFPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of central nervous system inflammation and B-cell proliferative diseases
Specimen Required
Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Send spinal fluid from collection vial 1.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| CSF | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 14 days |
Reference Values
0.70-1.80 mcg/mL
Day(s) Performed
Monday through Friday
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82232
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| B2MC | Beta-2-Microglobulin, CSF | 1951-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| B2MC | Beta-2-Microglobulin, CSF | 1951-3 |
Reject Due To
| Gross hemolysis | OK |
Method Name
Nephelometry
Method Description
In this Siemens Nephelometer II method, the light scattered onto the antigen-antibody complexes is measured. The intensity of the measured scattered light is proportional to the amount of antigen-antibody complexes in the sample under certain conditions. If the antibody volume is kept constant, the signal behaves proportionally to the antigen volume. A reference curve is generated by a standard with a known antigen content on which the scattered light signals of the samples can be evaluated and calculated as an antigen concentration. Antigen-antibody complexes are formed when a sample containing antigen and the corresponding antiserum are put into a cuvette. A light beam is generated with a light emitting diode, which is transmitted through the cuvette. The light is scattered onto the immuno-complexes that are present. Antigen and antibody are mixed in the initial measurement, but no complex is formed yet. An antigen-antibody complex is formed in the final measurement. The result is calculated by subtracting the value of the final measurement from the initial measurement. The distribution of intensity of the scattered light depends on the ratio of the particle size of the antigen-antibody complexes to the radiated wavelength.(Instruction manual: Siemens Nephelometer II, Siemens, Inc.; Version 3, 2008; Addendum to the Instruction Manual 2.3, 08/2017)