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HelpTest Code Epic LAB1086 Proinsulin, Plasma
Additional Codes
SQ code PRINSM
Mayo code PINS
Reporting Name
Proinsulin, PSpecimen Type
Plasma EDTAPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
As part of the diagnostic workup of suspected insulinoma
As part of the diagnostic workup of patients with suspected prohormone convertase 1/3 deficiency
As part of the diagnostic workup of patients with suspected proinsulin variations
Specimen Required
Patient Preparation:
1.Patient should fast for 8 hours before specimen collection.
2. Infants younger than 2 years should fast a maximum of 6 hours.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Ice-cooled, lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. After collection, place the whole blood on ice for at least 10 minutes, then centrifuge at refrigerated temperature.
2. Aliquot plasma into a plastic vial and send frozen.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 30 days |
Reference Values
3.6-22 pmol/L
Day(s) Performed
Tuesday, Friday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
84206
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PINS | Proinsulin, P | 27882-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 80908 | Proinsulin, P | 27882-0 |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Electrochemiluminescent Assay
Method Description
Sequential 2-site electrochemiluminescent immunoassay performed on the MesoScale Discovery instrument. The assay uses a monoclonal biotinylated anti-insulin capture antibody and an anti-C-peptide detection antibody labeled with SULFO-TAG. The signal is directly proportional to the amount of proinsulin in the sample. Assay calibration is traceable to the World Health Organization 1st International Standard for human proinsulin, NIBSC code: 09/296. This assay demonstrates no cross-reactivity with insulin or C-peptide.(Unpublished Mayo method)